Journal: Antibiotics

Article Title: Evaluation of the Inactivation of Microorganisms by a Blue Laser (445 nm)—An In Vitro Study

doi: 10.3390/antibiotics15020137

Figure Lengend Snippet: A box plot of the log 10 -transformed colony-forming unit (CFU) values of Enterococcus faecalis , Streptococcus mutans , and Candida albicans in untreated samples (negative controls), laser-treated samples (test groups 4 mm/s, 2 mm/s, and 1 mm/s), and positive controls treated with chlorhexidine digluconate (CHX). Data source .

Article Snippet: The test organisms Enterococcus faecalis (ATCC ® 29212TM) , Streptococcus mutans (DSM 20523/ATCC ® 25175TM), and Candida albicans (SC5314/ATCC MYA-2876D-5TM) were used for the main experiments: two Gram positive bacteria and one fungus as representatives of important dental pathogens.

Techniques: Transformation Assay

Journal: Antibiotics

Article Title: Evaluation of the Inactivation of Microorganisms by a Blue Laser (445 nm)—An In Vitro Study

doi: 10.3390/antibiotics15020137

Figure Lengend Snippet: Testing of the carrier solutions: phosphate buffered saline (PBS), deionised H 2 O, potassium phosphate buffer (KPO 4 ), sodium potassium phosphate buffer (NaKPO 4 ) and Tris-buffered saline with hydrochloric acid (Tris-HCl) by applying the microorganisms to the ceramic test specimens, vital staining using MTT, and subsequent microscopic evaluation of the microbial converted blue formazan. Tris-HCl reduced the viability slightly for Enterococcus faecalis and strongly for Streptococcus mutans . Sodium potassium phosphate buffer was not suitable for any of the test organisms. PBS was also not optimal for this test setup, with clearly recognisable salt crystals forming. Stable viability results were shown with the H 2 O (deionised) solution for Candida albicans and Enterococcus faecalis and with the potassium phosphate buffer solution for Streptococcus mutans . Figure source .

Article Snippet: The test organisms Enterococcus faecalis (ATCC ® 29212TM) , Streptococcus mutans (DSM 20523/ATCC ® 25175TM), and Candida albicans (SC5314/ATCC MYA-2876D-5TM) were used for the main experiments: two Gram positive bacteria and one fungus as representatives of important dental pathogens.

Techniques: Saline, Staining

Journal: Antimicrobial Resistance and Infection Control

Article Title: Tolerance of clinical vancomycin-resistant Enterococcus faecium isolates against UV-C light from a mobile source

doi: 10.1186/s13756-023-01259-3

Figure Lengend Snippet: Reduction of different clinical VRE isolates with UV-C doses of 50 (A) and 22 mJ/cm² (B) . The boxplots represent the reduction factors determined per tested VRE isolate (n = 10) and test organism E. hirae ATCC 10541 in 5 independent experiments, comparing microbial load on ceramic tiles before and after UV-C radiation with 50 (A) and 22 mJ/cm² (B) . Boxplots are in ascending order by median. Statistically significant results (p < 0.01) of pairwise Tukey post-hoc analysis are connected by brackets. Non-significant comparisons are not shown. The p-values are displayed as follows: p < 0.01 = *, p < 0.001 = **, p < 0.0001 = ***, and p < 0.00001 = ****

Article Snippet: Testing the efficacy of UV-C radiation for ten different whole-genome-sequenced clinical isolates of VRE as well as ATCC 10541 test organism Enterococcus hirae , showed differences in reduction.

Techniques: